TABLE 2.
Predicted effect of protein stability in the presence of amino acid mutations in the SARS-COV-2 genomes
| Protein | Mutation | ΔΔG SDM (kcal/mol) | ΔΔG DUET (kcal/mol) | Stability |
|---|---|---|---|---|
| Spike | A222V | 0.95 | 0.91 | Stabilizing |
| S477N | 0.31 | 0.02 | Stabilizing | |
| L18F | −0.801 | −0.46 | Destabilizing | |
| N439K | −0.29 | 0.3 | Inconclusive | |
| L5F | −0.801 | −0.1 | Destabilizing | |
| W1214G | −1.913 | −0.28 | Destabilizing | |
| R21I | −0.856 | 0.46 | Inconclusive | |
| A262S | −2.13 | −1.66 | Destabilizing | |
| S98F | 1.23 | −0.58 | Inconclusive | |
| D1163Y | 0.21 | 0.26 | Stabilizing | |
| G1167V | −0.58 | −2.25 | Destabilizing | |
| D936Y | −0.13 | −0.32 | Destabilizing | |
| P272L | 2.12 | 0.36 | Stabilizing | |
| D80Y | 0.77 | −3.08 | Inconclusive | |
| E583D | −0.69 | −0.86 | Destabilizing | |
| P1263L | −0.231 | 1.29 | Inconclusive | |
| K1073N | −0.48 | −0.45 | Destabilizing | |
| D253G | −0.19 | 0.04 | Inconclusive | |
| T723I | 0.64 | 0.21 | Stabilizing | |
| A688V | −0.18 | 0.03 | Inconclusive | |
| A626S | −2.6 | −1.66 | Destabilizing | |
| L54F | −0.61 | −1.33 | Destabilizing | |
| H655Y | 0.6 | 1.44 | Stabilizing | |
| G769V | 0.6 | 0.14 | Stabilizing | |
| L176F | 0.12 | −0.95 | Inconclusive | |
| G1124V | −1.52 | −0.14 | Destabilizing | |
| V622F | −0.2 | −0.67 | Destabilizing | |
| S255F | 0.94 | −0.8 | Inconclusive | |
| H49Y | 0.4 | 1.14 | Stabilizing | |
| D839Y | −0.389 | −1.08 | Destabilizing | |
| V1176F | −0.92 | −0.55 | Destabilizing | |
| D215H | 0.8 | 1.35 | Stabilizing | |
| H146Y | 1.139 | −0.29 | Inconclusive | |
| A879S | −2.57 | −1.69 | Destabilizing | |
| Q677H | 0.98 | −0.48 | Inconclusive | |
| D1084Y | −0.43 | −0.03 | Destabilizing | |
| V1068F | −1.05 | −1.15 | Destabilizing | |
| P25S | −0.392 | 0.93 | Inconclusive | |
| A520S | −1.23 | −0.15 | Destabilizing | |
| G261V | 0.16 | 0.16 | Stabilizing | |
| D574Y | −0.56 | −0.45 | Destabilizing | |
| T29I | 0.48 | 0.51 | Stabilizing | |
| Y453F | −0.17 | −0.48 | Destabilizing | |
| N501Y | 0.41 | −0.42 | Inconclusive | |
| S939F | 0.76 | −0.71 | Inconclusive | |
| T95I | 1.91 | 0.37 | Stabilizing | |
| Q675H | 0.8 | −0.4 | Inconclusive | |
| Nucleocapsid | A220V | −0.51 | −1.13 | Destabilizing |
| S194L | 1.15 | −0.02 | Inconclusive | |
| D103Y | 1.45 | 0.55 | Stabilizing | |
| P13L | 0.84 | 0.23 | Stabilizing | |
| S197L | 1.27 | 0.26 | Stabilizing | |
| A398V | −0.98 | −1.03 | Destabilizing | |
| P199L | 1.27 | −0.21 | Inconclusive | |
| M234I | 0.69 | 0.41 | Stabilizing | |
| S188L | 1.21 | −0.06 | Inconclusive | |
| S183Y | 0.05 | −0.71 | Inconclusive | |
| Membrane | T175M | 0.69 | −0.26 | Inconclusive |
| Envelope | P71S | −0.03 | −2.35 | Destabilizing |