Table 3.

Shows comparative lysis efficiencies of four different buffers at four different concentrations in the extraction of viral RNA from the saliva sample spiked with inactivated virus (5 × 10⁵ pfu/ml), the positive control, and the negative control.

Lysis Buffer	Sample type	Concentrations
		0.25%	0.50%	0.75%	1%
IGEPAL	Spiked saliva sample	Invalid	Invalid	Invalid	Invalid
	Positive control	Invalid	Invalid	Invalid	Invalid
	Negative control	Not applicable	Not applicable	Not applicable	Not applicable
Tween 80	Spiked saliva sample	Invalid	Invalid	Invalid	Invalid
	Positive control	Invalid	Invalid	Invalid	Invalid
	Negative control	Not applicable	Not applicable	Not applicable	Not applicable
Tween 20	Spiked saliva sample	Invalid	Invalid	Invalid	Invalid
	Positive control	Invalid	Invalid	Invalid	Invalid
	Negative control	Not applicable	Not applicable	Not applicable	Not applicable
Triton X 100	Spiked saliva sample	valid	valid	Invalid	Invalid
	Positive control	valid	valid	Invalid	Invalid
	Negative control	Not applicable	Not applicable	Not applicable	Not applicable

Invalid: sample changes colour from dark pink to orange/yellow before amplification.

Valid: sample changes colour from dark pink to orange/yellow after amplification.

Not applicable: sample does not change colour after amplification.