FIG 2.
Structural modeling of the M. tuberculosis Asn-transamidosome explains complex instability due to GatA-G444S. (A) Predicted structural model of the Asn-transamidosome. M. tuberculosis GatA, GatB, GatC, and ND-AspRS are colored magenta, green, cyan, and brown, respectively. tRNAAsn (yellow) docked onto the structure is from the P. aeruginosa asparagine-transamidosome (PDB ID 4WJ3). The site of the 444 residue of GatA is shown in the boxes. (B) GatA-G444S; (C) GatA-WT. G444 and S444 are colored red, while P56 from GatC is blue. The box shows a magnified 90° counterclockwise rotated view of the mutation site.