FIG 7.

Quantitation of IFN-α2, IFN-β, IFN-γ, CD8, PD-1, Tim-3, and CD80 RNA transcripts in TG of latently infected mice. Corneas from CD28^−/−, CD28^−/− CTLA4^−/−, PD-L1^−/−, and WT mice were scarified before ocular infection and infected ocularly with 2 × 10⁵ PFU per eye of KOS virus (as described for Fig. 1). On day 28 p.i., TG from infected mice were harvested, and qRT-PCR was performed on individual mouse TG. Expression of IFN-α2, IFN-β, IFN-γ, CD8, PD-1, Tim-3, and CD80 transcripts in TG of each infected mouse strain was normalized to the level of each transcript in the TG of their corresponding uninfected mouse strain (shown as fold increase or decrease compared to levels in their uninfected counterpart). GAPDH expression was used to normalize expression of each transcript in the TG of ocularly infected mice. Each bar represents the means ± SEM from CD28^−/− infected mice (20 TG), CD28^−/− CTLA4^−/− infected mice (18 TG), PD-L1^−/− infected mice (22 TG), and WT infected mice (12 TG). (A) IFN-α2 transcript; (B) IFN-β transcript; (C) IFN-γ transcript; (D) CD8 transcript; (E) PD-1 transcript; (F) Tim-3 transcript; (E) CD80 transcript.