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The C-terminal of vWbp harbors an Fg-binding site at residues 386 to 482. (A) Schematic overview of the vWbp constructs generated in this study. Gray represents the predicted unordered region; SP, signal peptide; D1D2, prothrombin-binding domain; Tag, N-terminal His6-MBP tag. (B) ELISA binding of soluble Fg to immobilized vWbp constructs (0.5 μg/well). (C) ELISA binding of vWbp constructs to immobilized Fg (0.5 μg/well).
