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. 2021 Aug 3;12(4):e01380-21. doi: 10.1128/mBio.01380-21

FIG 6.

FIG 6

Characterizations of TgStx10 and TgStx12. (A to C) TgStx12, TgStx10, and TgVAMP4-1 localized at the ELC. (D) TgStx12 and TgStx10 partially colocalized with TgTrs85. (E) Plaque assay examining the growth of 6HA-AID*-TgStx12 parasites in confluent HFFs after 8 days in the absence or presence of IAA. (F) Red/green invasion assay examining the ability of 6HA-AID*-TgStx12 parasites to attach to and invade host cells. The parasites were incubated for 6 h in the presence or absence of IAA before egression. (G) Replication of 6HA-AID*-TgStx12 parasites after growth in the presence or absence of IAA for 24 h. (H) The egress of 6HA-AID*-TgStx12 parasites from host cells was determined by IFA. Parasites were grown in HFFs for 20 h under normal conditions, and then IAA or ethanol was added for 16 h before stimulation with A23187. (I) Conditional ablation of TgStx12 exhibits bright fluorescence stained by TgSAG2 in the lumen of the partial parasitophorous vacuole. The parasites were treated with auxin for 60 h.