TABLE 2.
Plasmids and cosmid used in this study
| Cosmid or plasmid | Description | Reference or source |
|---|---|---|
| Cosmid 4P22 | S. venezuelae cosmid carrying cmlR and sven0904_0905 | Gift from M. Buttner |
| pIJ82 | Integrative cloning vector; ori pUC18 hyg oriT RK2 int ФC31 attP ФC31 | Gift from H. Kieser |
| pRT801 | Integrative cloning vector; ori pUC18 apra oriT RK2 int ФBT1 attP ФBT1 | 49 |
| pMS82 | Integrative cloning vector: hyg oriT int ФBT1 attP ФBT1 | 49 |
| pGUS | Integrative Streptomyces-specific reporter vector for transcriptional fusions with the gusA gene | 47 |
| pGUS-PermE* | Strong Streptomyces promoter, PermE*, upstream of gusA | R. J. St-Onge (unpublished) |
| pBluescript II KS(+) | Standard cloning vector | Stratagene |
| pET15b | Overexpression of N-terminally His6-tagged proteins | Novagen |
| pMC122 | cmlR cloned downstream of ermE* in pIJ82 | This study |
| pMC123 | cmlR cloned downstream of ermE* in pRT801 | This study |
| pMC124 | pET15b carrying cmlR for overexpression with an N-terminal His6 tag | This study |
| pMC125 | pMS82 carrying sven0904_0906 | This study |
| pMC126 | pGUS carrying sven0925_0926 | This study |
| pMC127 | pGUS-PermE* carrying promoterless sven0925_0926 | This study |