Table 2.
Primers and probes used in the study
| Primer/probe name | Target organism(s) | Target gene | Sequence |
|---|---|---|---|
| tsa47f | Orientia tsutsugamushi | tsa47 | TCCAGAATTAAATGAGAATTTAGGAC |
| tsa47r | TTAGTAATTACATCTCCAGGAGCAA | ||
| tsa47probe | [6FAM]TTCCACATTGTGCTGCAGATCCTTC[TAM] | ||
| Rick16Sf | Rickettsiaceae: Orientia tsutsugamushi/ Rickettsia canadensis | 16S | GCTACACGCGTGCTACAATGG |
| Rick16SRr | TGTGTACAAGGCCCGAGAACG | ||
| Rick16Sprobe | [6FAM] ATCGCTAGTAATCGCGGATCAGCATGCC [TAM] | ||
| Ana16Sf | Anaplasmataceae: Wolbachia/Anaplasma/Ehrlichia | 16S | ACTGGAGGAAGGTGGGGATG |
| Ana16Sr | TGATCCACGATTACTAGCGATTCC | ||
| Ana16Sprobe | [6FAM] TGGGCTACACACGTGCTACAATGG [TAM] |