FIG 7.

Reovirus mRNAs are recruited to factory-like structures by σNS. Cells were transfected with expression plasmids encoding μNS, σ3, and the σNS constructs shown and incubated for 24 h. Cells were fixed, stained using RNA FISH probes specific for σNS mRNA (white) or σ3 mRNA (yellow), μNS-specific antiserum (magenta), and DAPI (blue), and imaged using confocal microscopy. Bar, 4.8 μm. The percentage of cytoplasmic σNS-mRNA (B, white bars) and σ3-mRNA (B, yellow bars) FISH signals in factory-like structures was quantified by dividing the sum of the FISH signal in factory-like structures by the sum of the cytoplasmic FISH signal. Individual data points represent single cells. Percentage values that differ significantly from those obtained from cells cotransfected with WT σNS, σ3, and μNS by one-way analysis of variance (ANOVA) with Dunnett’s multiple-comparison test are shown. ****, P < 0.0001.