TABLE 2.
Binding affinities of TlpALBD and TlpALBD membrane-distal and -proximal dCache mutants for TlpA ligandsa
| Ligand | Mean binding affinity (μM) ± SD |
|||||
|---|---|---|---|---|---|---|
| TlpAWT | TlpAD165A | TlpAM183A | TlpAY228A | TlpAY252A | TlpAD254A | |
| Arginine | 2 ± 0.11 | 12.4 ± 0.84 | 22.3 ± 5.9 | 8.3 ± 1.6 | 4.96 ± 1.8 | 3.71 ± 0.73 |
| Cysteine | 4.7 ± 0.3 | 41.9 ± 0.4 | 36.1 ± 15.7 | 15.7 ± 6.6 | 5.5 ± 0.8 | 4.58 ± 1.5 |
| Fumarate | 10 ± 1.5 | 100.5 ± 38.9 | 106.1 ± 36.6 | 46.6 ± 3.9 | 8.5 ± 1.7 | 44 ± 8.1 |
| Glucosamine | 10.5 ± 2.8 | 96 ± 24.3 | 94.6 ± 57.3 | 17.1 ± 70 | 2.96 ± 0.7 | 9.84 ± 3.7 |
| Malic acid | 46 ± 17 | 11.9 ± 4.3 | 33.6 ± 6.2 | 28.6 ± 9.4 | 14.2 ± 6.2 | 29.3 ± 2 |
| Thiamine | 60 ± 0.6 | 43.9 ± 14.1 | 18.1 ± 1.2 | 29.5 ± 12.3 | 35 ± 7.3 | 39.3 ± 4.3 |
| α-Ketoglutarate | 224 ± 11.2 | 124 ± 7.1 | 256.2 ± 27.6 | 295.4 ± 27.7 | 289 ± 22.6 | 321 ± 21.6 |
a
Data represent the mean values ± SD from three independent experiments (n = 3).