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. 2021 Jul 6;12(4):e02531-20. doi: 10.1128/mBio.02531-20

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Copyright © 2021 Rissanen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Immunization with HTNV Gn elicits a nAb response enabling isolation of neutralizing mAb HTN-Gn1 and non-neutralizing mAb nnHTN-Gn2. (A) Analysis of the IgG-specific response to HTNV Gn by ELISA in rabbit sera (rabbits 3946 to 3949) following the second (blue, indicated by _2) and third (black, indicated by _3) HTNV Gn immunizations. A prebleed serum control is shown in green. (B) Neutralization of live HTNV strain 76-118 by rabbit sera (rabbits 3946 to 3949) following the third HTNV Gn immunization. A prebleed serum control is shown in green. (C) Characterization of mAbs HTN-Gn1 and nnHTN-Gn2 binding to HTNV Gn by ELISA. (D) Neutralization of live HTNV strain 76-118 by mAbs HTN-Gn1 and nnHTN-Gn2. Despite mAb nnHTN-Gn2 exhibiting high binding to HTNV Gn, this mAb did not show neutralizing activity. Error bars represent the standard errors of the mean. In panels A and C, ELISA was carried out three times in duplicate. In panels B and D, the neutralization assay was carried out twice in duplicate. Representative graphs are shown.