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. 2021 Jul 6;12(4):e01871-20. doi: 10.1128/mBio.01871-20

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Copyright © 2021 Bell et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — TAX1BP1’s coiled-coil domain and galectin-8’s CRD2 are required for their interaction. (A) Schematic representation of TAX1BP1 domain structure and truncations used in panels B and C. CLIR, noncanonical/LC3C-interacting region; UBZ, ubiquitin-binding zinc finger domain; CC, coiled-coil domains; oligo, oligomerization domain. (B and C) Directed coimmunoprecipitations (IP) of 3×FLAG (FL)-tagged galectin-8 ectopically expressed in HEK293Ts. Whole-cell lysates (WCL) and co-IPs probed for HA-tagged TAX1BP1 truncations. HA-GFP shown as negative control for interaction. (D) Schematic of galectin-8 domain structure and truncations. CRD, carbohydrate recognition domain. (E) Directed co-IPs of HA-TAX1BP1 expressed in HEK293T cells. WCLs and co-IPs probed for FL-galectin-8 truncations. (F) Same as in panels B and C but with mouse (ms) and human (hu) FL-galectin-8, HA-TAX1BP1, and HA-NDP52.