FIG 1.

CCL5 directs ERK1/2 survival signaling responses in ZIKV-infected hBMECs. (A) Primary hBMECs were infected with ZIKV (PRVABC59) at an MOI of 10 or mock infected, and at 3 and 9 dpi, supernatants were analyzed by an ELISA (R&D Systems) for CCL5/RANTES relative to antigen standards. (B to D) hBMECs were starved overnight and CCL5 treated (100 ng/ml) (B) or mock or ZIKV infected at 3 and 9 dpi (C and D). Protein levels of ERK1/2, P-ERK1/2, AKT, P-AKT, and GAPDH controls were determined by Western blotting. Asterisks indicate statistical significance (*, P < 0.05; ***, P < 0.001). Experiments were performed at least 3 times with similar results.