FIGURE 6.

cGAMP MPs protect WT mice from EAE by IL-27–dependent IL-10 production. (A) Soluble (5 μg) or equivalent cGAMP MPs were injected i.m. into mice at days 9, 11, and 13 after EAE induction. Samples from mice were obtained at 6, 12, 24, and 48 h after final injection and evaluated for local cytokine production (n = 6). (B–D) WT mice were induced to EAE and injected i.m. with 5 μg cGAMP in MPs or equivalent levels of blank MP or cGAMP controls at days 9 and 11. Samples were collected on day 12. No EAE represents healthy control mice. Percentage and total number flow cytometry analysis of IL-10–producing CD4⁺T cells and CD11c⁺ DCs after PMA/ionomycin stimulation. Data from two experiments (n = 6–10). (E) WT, Ifnar^−/−, Il27ra^−/−, or Il10^−/− mice were induced to EAE and injected i.m. with 5 μg cGAMP MPs on days 9, 11, 13, 15, and 17 and sacrificed at day 28. (E) Disease scores of two combined experiments per genotype (n = 7–10). Significance between groups was determined by two-way ANOVA. All other statistics were evaluated by Mann–Whitney U tests. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.