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SENP3 localization in the nucleus and cytoplasm and accumulation within the cells upon DEN exposure (A,B) LO2 cells were treated with DEN for an indicated time period, and then DN damage and SENP3 level were analyzed by western blot analysis. (C) LO2 cells were treated with DEN for 2 h, and then SENP3 was visualized by immunofluorescence and distinguished between nuclear localization and cytoplasmic localization with different methods of permeabilization. Scale bar: 100 μm.
