Figure 2.

BioID of SENP3-interacting proteins by SENP3–BirA   (A) Schematic workflow of the BioID method with SENP3–BirA. (B) HEK293T cells were transfected with the indicated plasmids. At 24 h after transfection, the expression levels of SENP3–BirA and control BirA were determined. Biotinylation of SENP3-interacting proteins was determined in the presence or absence of biotin pretreatment for 24 h. (C) LO2 cells were transfected with pCDH–SENP3–BirA or FLAG–SENP3. SENP3 was precipitated with indicating methods and then nucleophosmin (NPM1) was detected.