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. 2021 May 25;22(7):e51530. doi: 10.15252/embr.202051530
Product name Vendor Catalog Number / Size
Bovine serum albumin (BSA) Cell Signaling Technology 9998S / 50 g
Dextran Sulfate sodium salt (from Leuconostoc spp.) Sigma‐Aldrich D8787 / 5 g
Formaldehyde, 16% Solution (w/v), Methanol‐free ThermoFisher Scientific 28906 / 10 × 1 ml ampules
**Formamide, ReagentPlus, ≥ 99.0% (GC) Sigma‐Aldrich F7503 / 250 ml
Heptane, ReagentPlus, 99% Sigma‐Aldrich H2198 / 1 l
Normal Goat Serum (NGS) Cell Signaling Technology 5425S / 10 ml
NP‐40 (Tergitol), 70% in H2O Sigma‐Aldrich NP40S / 100 ml
Phosphate‐buffered saline (PBS) Quality Biological 119‐069‐131 / 1000 ml
Polyethylene glycol (PEG‐8000) Promega V3011 / 500 g
Potassium chloride Sigma‐Aldrich PHR1329 / 5 g
Potassium phosphate monobasic Sigma‐Aldrich P0662 / 25 g
Schneider’s Insect Medium, 1× [+] L‐Glutamine Life Technologies (Gibco) 21720‐024 / 500 ml
Sodium chloride ThermoFisher Scientific BP358‐1 / 1 kg
Sodium phosphate monobasic monohydrate BioXtra for molecular biology, ≥ 99.5% (T) Sigma‐Aldrich 71507‐250G / 250 g
SSC, 20× Quality Biological 351‐003‐101 / 500 ml
TritonX‐100 Sigma‐Aldrich T8787 / 100 ml
Tween20 Sigma‐Aldrich P9416 / 50 ml