FIGURE 2.

ML‐385 retarded Mel‐induced Nrf2 nuclear translocation and antioxidative effects but had little effect on AMPK activation in myocardial I/R injury. A. Representative immunoblots of p‐AMPK, AMPK, nuc‐Nrf2, Histone3 (internal control), cyto‐Nrf2 and GAPDH (internal control): A. sham; B. I/R; C. Mel + I/R; D. Mel + CC + I/R; B‐D. Semi‐quantitative analysis of p‐AMPK, nuc‐Nrf2 and cyto‐Nrf2; E. MDA contents; F. SOD activity; G. DHE intensity. Data are presented as mean ± SEM, n = 6. I/R: ischaemia/reperfusion; Mel: melatonin; ML: ML‐385; nuc‐Nrf2: intranuclear Nrf2; cyto‐Nrf2: cytoplasmic Nrf2. ^** P < .01 vs. the sham group, ^## P < .01 vs. the I/R group, ^^P < .01 vs. the Mel + I/R group