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. 2021 Jun 17;25(13):6046–6055. doi: 10.1111/jcmm.16463

FIGURE 5.

FIGURE 5

AKT pathway is essential in TSP4‐regulated pro‐invasive function. A, Incubation of T24 cells with recombinant TSP4 protein at the indicated time points, p‐AKT and total AKT protein expression were evaluated using a western blot assay. B, Pretreatment of T24 cells with AKTi (0.5 μmol/L) for 30 min followed by with or without TSP4 (30 ng/mL) for 24 h. The MMP2 protein levels were evaluated using a western blot assay. C and D, T24 cells were treated with AKTi (0.5 μmol/L) for 30 min, followed by TSP4 (30 ng/mL) for 24 h. Cell migration and invasion were measured using a Transwell assay. The cell numbers of migrating and invading cells were quantified. All data are expressed in terms of the mean ± the SD of triplicate samples. ****P < .0001 relative to the control group. #### P < .0001 relative to the TSP4‐treated group