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. 2021 Jun 19;11(7):1997–2007. doi: 10.1002/2211-5463.13207

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© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Differences between mitochondria in A459 parental and A549‐SD stem cells. (A) The ROS levels of A549 and A549‐SD cells. (Bi) The mitochondrial membrane potential of A549 cells. (Bii) The mitochondrial membrane potential of A549‐SD cells. (C) Distribution and size of mitochondrial in A549 and A549‐SD cells. Mitochondrial protein TOMM20 was used as a marker of mitochondrial. Scale bar = 3 µm. (Di) Sizes of 100 single mitochondria in A549 and A549‐SD cells. (Dii) The average size of 100 mitochondria in A549 and A549‐SD cells. (E) Expression of mitochondrial fission and fusion‐related genes in A549 and A549‐SD cells. *P < 0.05, **P < 0.01; one‐way analysis of variance; the error bars indicate the SD; the number of biologically‐independent replicates is 3.