Fig. 4.

Mitochondrial division promotes the stemness of tumor stem cells. (A) Expression of stemness genes after sh‐mediated silencing of Fis1. (B) Spheroid formation rate of a single cell in a 96‐well plate after sh‐mediated silencing of Fis1. (C) Spheroid formation of 1000 cells in a six‐well plate after sh‐mediated silencing of Fis1. Scale bar = 300 μm. (Di) Tumors taken from nude mice 2 weeks after injection with A549‐SD‐sh‐NC and A549‐SD‐sh‐FIS1 cells. Scale bar is 1 cm. (Dii) Tumor weight of A549‐SD‐sh‐NC and A549‐SD‐sh‐FIS1 tumors. **P < 0.01, ***P < 0.001; one‐way analysis of variance; the error bars indicate the SD; the number of biologically‐independent replicates is 3.