FIGURE 2.

FIGURE(A,B) The growth inhibitory effects of 6‐shogaol and 10‐gingerol on four different leukemic cell lines. Human leukaemic cell lines were treated with increasing concentrations of 6Sh and 10‐gingerol for different time points related to their growth curves. The percentage of cell viability was decreased in a dose‐dependent manner, especially for Nalm‐6 and R‐CCRF‐CEM cell lines. Among these two cell lines, Nalm‐6 was chosen for subsequent experiments since the related immunophenotype was more prevalent among children with ALL. Furthermore 200 µmol/L 6Sh was selected due to its proximity to the half‐maximal concentrations of the proliferation inhibition (IC50) of 6Sh on Nalm‐6 cell line. (C) Neither 6‐shogaol (6Sh), nor its solvent affect the survival of normal mononuclear cells (MNCs). Mononuclear cells were isolated from three volunteers. 10 × 10⁴ cells were seeded per well. Following 48 h treatment with 0.4% DMSO or 200 µmol/L 6Sh, the viability of MNCs was determined using MTT assay. For the control group, cells were incubated with RPMI1640 alone. Data were reported as mean ± SEM of three separate experiments. Each experiment was performed in triplicates. Black = CCRF‐CEM cell line, blue = R‐CCRF‐CEM cell line, green = Nalm‐6 cell line and red = RN95 cell line