TABLE 1.
Primer sequences of PUMA, FASN and GAPDH
| Gene | Primer sequence (5' to 3') | Primer length (base pairs) | Amplicon length (base pairs) | T m | GC% |
|---|---|---|---|---|---|
| PUMA | F: GACCTCAACGCACAGTACGAG | 21 | 98 | 60.99 | 57.14 |
| R: AGGAGTCCCATGATGAGATTGT | 22 | 58.61 | 45.45 | ||
| FASN | F: CCGCTTCCGAGATTCCATCCTACGC | 25 | 137 | 67.13 | 60 |
| R: GGATGGCAGTCAGGCTCACAAACG | 24 | 66.19 | 58.3 | ||
| GAPDH | F: GCCCCAGCAAGAGCACAAGAGGAAGA | 26 | 106 | 68.64 | 57.69 |
| R: CATGGCAACTGTGAGGAGGGGAGATT | 26 | 66.38 | 53.85 |
Abbreviations: F, forward; FASN, fatty Acid Synthase; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; PUMA, p53 upregulated modulator of apoptosis; R, reverse; Tm, melting temperature.