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Estrogens induce TGIF1 expression in ZZ gonads.TGIF1 WISH was performed on E9.5 male and female urogenital tissue treated in ovo with 17β-estradiol (E2) or vehicle (Control). Tissues were sectioned and immunofluorescence for aromatase (pre-granulosa marker) or AMH (Sertoli cell marker) and cytokeratin (cortical marker) was performed to evaluate the efficacy of the sex reversal.
