Fig. 4.

Estrogen synthesis inhibition by fadrozole results in downregulation of TGIF1 in ZW gonads. (A) TGIF1 WISH was performed on E9.5 male and female urogenital tissue treated in ovo with the aromatase inhibitor fadrozole (AI) or vehicle (Control). Tissues were sectioned and immunofluorescence for aromatase (pre-granulosa marker) or AMH (Sertoli cell marker) and cytokeratin (cortical marker) was performed to evaluate the efficacy of the sex reversal. (B) TGIF1 qRT-PCR was performed in gonadal samples of E9.5 embryos treated with the aromatase inhibitor (AI) or vehicle (Control). Expression level is relative to β-actin and normalized to the male control. Bars represent mean±s.e.m., n=6. **adjusted P<0.01. Multiple t-tests and Holm–Sidak post-hoc test.