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. 2021 Aug 23;148(16):dev199646. doi: 10.1242/dev.199646

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — TGIF1 overexpression in left male gonads results in gonadal feminization. TOL2-TGIF1 overexpression (TGIF1 OE) or control (GFP Control) plasmids were electroporated in male left E2.5 coelomic epithelium. Gonads were examined at E8.5. (A-C) Immunofluorescence against cytokeratin (epithelial/cortical marker; A), fibronectin (interstitial cell marker; B) and AMH (Sertoli cell marker; C) was performed in transverse sections. Dashed box indicates the magnified area. Dotted line delineates the gonadal epithelium. White arrow indicates the epithelium (E) or JCM, white arrowhead indicates EMT-derived interstitial cell and M indicates medulla. (D) Quantification of the percentage of epithelial area, relative to the total gonadal area in male gonads. (E) Quantification of the average epithelium thickness (in μm) in control or TGIF1-overexpressing male gonads. (F) Quantification of the percentage of JCM area, relative to the total medullar area. Bars represent mean±s.e.m., n≥6. Unpaired two-tailed t-test. *P<0.05; ***P<0.001.