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. 2021 Aug 23;148(16):dev199646. doi: 10.1242/dev.199646

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — TGIF1 overexpression in right female gonads results in epithelial thickening and JCM formation. TOL2 TGIF1 overexpression (TGIF1 OE) or control (GFP Control) plasmids were electroporated in female right E2.5 coelomic epithelium. Gonads were examined at E8.5. (A-C) Immunofluorescence against cytokeratin (epithelial/cortical marker; A), fibronectin (interstitial cell marker; B) and aromatase (pre-granulosa cell marker; C) was performed in transverse sections. Dashed box indicates the magnified area. Dotted line delineates the gonadal epithelium. White arrow indicates the epithelium (E) or the JCM. (D) Quantification of the average epithelium thickness (in μm) in control or TGIF1-overexpressing female gonads. (E) Quantification of the percentage of JCM area, relative to the total medullar area. Bars represent mean±s.e.m., n=3. Unpaired two-tailed t-test. *P<0.05.