Fig. 6.

OZ treatment inhibits SAH-induced NF-κB p65 activation. a, b Western blot analysis of p-IκBa, IκBa in treated mice and primary neurons. OZ treatment significantly restrained the phosphorylation of IκBa and the degradation of IκBa induced by SAH. c, d Nuclear fractions of brain tissue and neurons were extracted. Protein levels of nuclear p-NF-κB p65 and NF-κB p65 in treated mice and primary neurons. OZ administration reduced SAH-induced enhancement of nuclear p-NF-κB p65. e, f mRNA levels of NLRP3, IL-β, and IL-18 in treated mice and primary neurons. Data are expressed as mean ± SD, n = 5 per group. ***P < 0.001 vs Sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs SAH+Vehicle group. ^†††P < 0.001 vs Control group; ^§P < 0.05, ^§§P < 0.01, ^§§§P < 0.001 vs OxyHb group