Fig. 7.

Effect of TAK1 knockdown on brain injury and inflammatory response after SAH. To knockdown of TAK1 in vivo, SAH mice were i.c.v injected with TAK1 siRNA. a Colocalization of Cy5-conjugated TAK1 siRNA with neurons (NeuN) at 24 h after SAH. b Western blotting and quantitative analysis for p-TAK1 and TAK1. c Modified Garcia score, d Beam balance test, and e brain water content in Sham, SAH+Vehicle, SAH+Scr siRNA, and SAH+TAK1 siRNA groups at 24 h after SAH. f–i Immunoblotting analysis and quantitation for NLRP3, ASC, cleaved caspase-1, IL-1β, GSDMD, nuclear p-NF-κB p65, and NF-κB p65 in treated mice. Data are expressed as mean ± SD, n = 5 in each group. ***P < 0.001 vs Sham group; ^##P < 0.01, ^###P < 0.001 vs SAH+Scr siRNA group. N.S., no significant difference. Scale bar: 50 μm