Table 2 -.
Single-cell and spatial technologies sample requirements and data analysis opportunities.
| TECHNOLOGY | Single-cell proteomics | Single-cell transcriptomics | Spatial proteomics | Spatial transcriptomics | ||
|---|---|---|---|---|---|---|
| scRNA-seq | snRNA-seq | Slide-seq, 10× Genomics | SeqFISH, SeqFISH+, MERFISH | |||
| Sample | Dissociated cells | Viable dissociated cells | Viable nuclei (fresh or frozen tumor) | Frozen, FFPE | Frozen | Frozen, FFPE |
| Tissue type | Tumor and normal tissue, blood | Tumor and normal tissue | ||||
| Genome coverage | Pre-selected markers (~40 markers) |
Genome-wide | Pre-selected markers (~40 markers) |
Genome-wide | Pre-selected markers (up to 10,000 genes) |
|
| DATA ANALYSIS | ||||||
| Cell composition | Yes | |||||
| Cell states | Yes | |||||
| Differential expression | Yes | |||||
| Pathway analysis | No | Yes | No | Yes | ||
| Cell fate trajectories | No | Yes | No | |||
| Molecular interaction | No | Yes | No | Yes | ||
| Cellular interactions | No | Yes | ||||
| scTCR/scBCR | No | Yes | No | |||