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. 2021 Aug 12;11:677646. doi: 10.3389/fonc.2021.677646

Figure 5.

Figure 5

HIST2H2BF promoted tumor initiation, self-renewal, and liver metastasis in mice. (A) LOVO and SW480 cells transfected with lentivirus HIST2H2BF or shHIST2H2BF were injected subcutaneously in nonobese diabetic/severe combined immunodeficiency mice. The tumor-forming rates were determined 2 months post-injection. (B) The frequency of CSCs was obtained. (C) Tumors harvested from LOVO and SW480 cells, with or without cisplatin treatment. (D) Growth curves of LOVO and SW480 xenografts. Two-way ANOVA was employed to analyze the differences in tumor growth. (E) Tumor weight was weighted in LOVO and SW480 cells. (F) Ki-67 and TUNEL staining in the tumors harvested from LOVO and SW480 cells without cisplatin treatment. (G) Representative images of liver metastatic foci (marked by black arrowheads). (H) IHC staining for HIST2H2BF expression in the liver metastatic foci from LOVO and SW480 cells. (I) Hematoxylin and eosin (HE) of liver metastasis in the mouse model (upper panel). Scale bar, 200 μm. The number of liver metastatic foci were counted (lower panel). (J) OS of mice injected with LOVO and SW480 cells. *P < 0.05, **P < 0.01, ***P < 0.001.