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. 2021 Aug 12;12:703711. doi: 10.3389/fmicb.2021.703711

FIGURE 1.

FIGURE 1

Eleven breeding sites and five controls were set up using a plastic cup filled with type I water (day 1) plus sterilized food solution (day 2). Treatment 1 (green) cups served as experimental controls. Treatment 2 (yellow) breeding sites were visited by an ovipositing female, individually. Treatment 3 (purple) breeding sites were set up with manually deposited eggs. Controls were collected on day 14. Breeding site water samples that had larvae developing were collected when at least one pupa was observed.