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. Author manuscript; available in PMC: 2022 Aug 3.
Published in final edited form as: Chembiochem. 2021 Jun 21;22(15):2530–2534. doi: 10.1002/cbic.202100218

Figure 1.

Figure 1.

Genetic incorporation of BzK into proteins in E. coli using the evolved Ma-tRNAPyl/MaBzKRS. (a) Structure of BzK. (b) Structure of M. alvus PylRS amino acid binding pocket with residues mutated in the library shown in stick. To illustrate the position of the bound Pyl, M. alvus PylRS apo form (PDB 6JP2) was superimposed on M. mazei PylRSc bound with Pyl (PDB 2ZCE). (c) SDS-PAGE analysis of WT Ub and Ub(6BzK) expressed and purified from E. coli. (d) ESI-TOF MS spectrum of the intact Ub(6BzK).