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. Author manuscript; available in PMC: 2022 Aug 3.
Published in final edited form as: Chembiochem. 2021 Jun 21;22(15):2530–2534. doi: 10.1002/cbic.202100218

Figure 3.

Figure 3.

Genetic incorporation of BzK into proteins in mammalian cells. (a) Flow cytometric analysis of BzK incorporation into EGFP(182TAG) in HEK-293T cells. Cells were co-transfected with pNEU-BzKRS and pcDNA3.1-EGFP(182TAG) and cultured with or without BzK for 24–48 h. (b) Fluorescence confocal microscopic analysis of BzK incorporation into EGFP(182TAG) in HEK-293T cells. (c) Western blot analysis of BzK incorporation into EGFP(182TAG) in HEK-293T cells. An anti-GFP antibody was used to detect EGFP, and an anti-benzoyllysine antibody was used to detect BzK incorporated into EGFP protein.