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p53 immunopurified from SV40-transformed cells copurifies with T antigen. (A) Silver-stained SDS-polyacrylamide gel of the p53-T antigen complex purified from four SV40-transformed cell lines derived from mouse (SCID and SV-T2), human (WI38 VA13), and monkey (COS-7) cells. (B) Western blot of the p53-T antigen complex, using anti-T antigen antibody Pab 108, indicating that two forms of T antigen (regular T antigen and super T) are present in the mouse cell lines.
