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. 2021 Aug 31;40:274. doi: 10.1186/s13046-021-02083-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — SHOX2 directly activates WASF3 gene transcription. (a) The positive correlation between the expression of SHOX2 and three WASF genes illustrated in scatter plot from the TCGA breast cancer cohort. (b) The effect of SHOX2 overexpression on WASF gene expression in T47D cells determined by QRT-PCR. (c, d) The effect of SHOX2 gene manipulations on WASF3 expression in T47D (c) and MDA-MB-231 (d) cells determined by Western blot. (e) The protein levels of WASF3 in xenograft breast tumors derived from SHOX2 knockdown and the knockdown control MDA-MB-231 cell. Representative images and quantitative data (n = 5 mice/group) are shown in the left and right panels, respectively. (f, g) The effect of SHOX2 gene manipulations on the luciferase activity in T47D (f) and MDA-MB-231 (g) cells transfected with the constructs harboring different lengths of the WASF3 promoters. ^*p < 0.05, ^**p < 0.01