Fig. 2.

CircRNF13 inhibits the proliferation, migration, and invasion of NPC. A Overexpression of circRNF13 inhibited, while knockdown of circRNF13 promoted proliferation of HNE2 and CNE2 cells, as assessed using MTT assay. Data have been represented as mean ± SD. *, p < 0.05; **, p < 0.05; ***, p < 0.001. B Clone formation assay showed that overexpression of circRNF13 inhibited the colony formation capability of HNE2 and CNE2 cells. Data have been represented as mean ± SD. ***, p < 0.001. C CircRNF13’s function in the tumor cell cycle was assessed using flow cytometry. HNE2 and CNE2 cells were stained with PI, after overexpression or knockdown of circRNF13. D CircRNF13 inhibited the migration ability of HNE2 and CNE2 cells transfected with sicircRNF13 or circRNF13 overexpression vector, as assessed using wound-healing assay. Images were acquired at 0 and 24 h. Data have been represented as mean ± SD. *, p < 0.05; ***, p < 0.001. E CircRNF13 inhibited the invasive ability of HNE2 and CNE2 cells after knockdown or overexpression of circRNF13, as assessed using Transwell invasion assays. Images were acquired at 24 h, and are representative of three independent experiments. Data have been represented as mean ± SD. **, p < 0.01; ***, p < 0.001