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. 2021 Aug 31;20:112. doi: 10.1186/s12943-021-01409-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — CircRNF13 inhibits glycolysis in NPC cells by promoting GLUT1 ubiquitination. A The mRNA levels of GLUT1, HK2, and LDHA in HNE2 and CNE2 cells were examined using RT-PCR, upon overexpression and knockdown of circRNF13. Data have been represented as mean ± SD; ns, no significance; ***, p < 0.001. B The expression levels of GLUT1, HK2, and LDHA proteins were examined using western blotting in HNE2 and CNE2 cells, upon overexpression and knockdown of circRNF13. C Degradation of GLUT1 was detected in HNE2 and CNE2 cells using western blotting, after overexpression and knockdown of circRNF13 and treatment with 50 μg/mL cycloheximide (CHX). D Ubiquitination levels of GLUT1 protein were detected in HNE2 and CNE2 cells using pull-down with an anti-Flag antibody and western blot with an anti-ubiquitin antibody, after overexpression or knockdown of circRNF13