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. 2021 Aug 31;20:112. doi: 10.1186/s12943-021-01409-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — CircRNF13 directly binds to and stabilizes SUMO2 mRNA, to upregulate its expression A. Expression of SUMO2 protein was examined using western blotting in HNE2 and CNE2 cells, after overexpression and knockdown of circRNF13. B Expression of SUMO2 mRNA was examined in HNE2 and CNE2 cells using RT-PCR, after overexpression and knockdown of circRNF13. C Bioinformatics prediction using the RNAhybrid website revealed that circRNF13 has a continuous binding site with the 3′-UTR of SUMO2 mRNA. D circRNF13 was found to bind to the 3′-UTR of SUMO2 mRNA in HNE2 and CNE2 cells using RNA pull-down assay. GADPH was used as a negative control. Data have been represented as mean ± SD. ***, p < 0.001. EcircRNF13 enhanced the luciferase activity of the SUMO2 mRNA 3′-UTR in HNE2 and CNE2 cells, as assessed using dual luciferase reporter assay. The 3′-UTR of GADPH was used as a negative control. Data have been represented as mean ± SD. *, p < 0.05; **, p < 0.01. F. Degradation of SUMO2 was detected in HNE2 and CNE2 cells using RT-PCR, after overexpression and knockdown of circRNF13 and treatment with 2 μg/mL actinomycin D. Data have been represented as mean ± SD. ***, p < 0.001, ****, p < 0.0001