Figure 1.

PTK7-CAR generation, cell-surface expression, and transduction of human T cells. (A) PTK7-CAR was generated by fusing PTK7-specific scFv to the co-stimulatory signaling domain of the 4-1BB (BB-ICD) and activating signaling domain of CD3ζ (CD3ζ-ICD), a T2A ribosomal skipping sequence, and tEGFR was included for the detection of CAR-modified T cells. (B) 293T cells transfected with control or PTK7-CAR constructs express both CAR and the marker gene tEGFR. (C) PTK7-CAR expression on transduced human CD4⁺ and CD8⁺ T cells was approximately 40–60% and 20–30%, respectively, as determined by tEGFR and CAR co-staining.