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. 2021 Aug 12;12:665970. doi: 10.3389/fimmu.2021.665970

Figure 5.

Figure 5

PTK7-CAR2 T cells retain effector function upon recursive target exposure. (A) Schematics of the long-term cytotoxicity assay. (B) Counts of H520 and H446 target cells after each round of recursive co-culture (rounds 1–3, R1–R3) with control or PTK7-CAR T cells. (C) Counts of control or PTK7-CAR T cells after each round of recursive co-culture with target cells. (D) Intracellular staining for granzyme B of control or PTK7-CAR T cells at the end of round 1 and 3 co-culture with H520 tumor cells. (E) PD-1 expression in control or PTK7-CAR T cells after rounds 1 and 3 of recursive co-culture with H520 tumor cells. (F) Percentage of PD-1+TIM-3+ cells in control or PTK7-CAR T cells after rounds 1 and 3 of recursive co-culture with H520 tumor cells. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, determined by repeated-measures two-way ANOVA with Tukey’s post hoc test.