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PTK7-CAR2 T cells do not mediate detectable on-target off-tumor toxicity. Control or PTK7-CAR T cells were tested reactivity against a panel of primary human normal epithelial cells or HUVECs in the cytotoxicity assays at the indicated effector-to-target ratios. Shown are mean ± SEM of % cell killing in triplicate wells. **P < 0.01, determined by repeated-measures two-way ANOVA with Tukey’s post hoc test.
