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. 2021 Aug 12;15:710820. doi: 10.3389/fncel.2021.710820

FIGURE 2.

FIGURE 2

Effect of CBX on MPP+-induced apoptosis in cultured astrocytes. (A,B) The effect of different doses of MPP+ (0, 50, 100, 500, and 1,000 μM) on the expression of caspase 3 and Panx 1 by qPCR assay. (C,D) western blotting analysis of Panx 1 expression after treatment with 500 μM MPP+. Protein levels were quantified and normalized to the GAPDH levels. (E–H) Influence of CBX (100 μM) on the expression of caspase 3 and selected inflammatory cytokines IL-1β, IL-6, and TGF-β in MPP+-induced astrocyte apoptosis. The data were normalized to 18S mRNA levels, n = 3. All data are mean ± SEM. Statistical comparison for (A,B,E–H) were performed using one-way ANOVA, following by Newman–Keuls Multiple Comparison Test, and for (D) using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control group (0 μM MPP+, #p < 0.05 compared with the group of 500 μM MPP+ alone).