FIGURE 3.

Effect of Panx 1 downregulation on MPP⁺-induced apoptosis in primary cultured astrocytes. (A–C) The silencing efficiency of three Panx 1-targeting siRNA was determined by qPCR and western blot analysis in primary cultured astrocytes. (D,E) qPCR analysis of Panx 1 expression in negative control and siRNA groups treated with 500 or 1,000 μM MPP⁺. (F–M) Effect of Panx 1 downregulation on the expression of selected inflammatory cytokines, including IL-1β, IL-6, TNF-α, and TGF-β in MPP⁺-induced astrocytes. (N,O) Influence of Panx 1 siRNA on caspase 3 expression in MPP⁺- induced astrocytes. The data were normalized to GAPDH or 18S mRNA levels, n = 3. All data are mean ± SEM. Statistical comparison for (A,C) were performed using one-way ANOVA, following by Newman–Keuls Multiple Comparison Test, and for (D–O) using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001 compared with negative group.