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. 2021 Aug 12;15:710820. doi: 10.3389/fncel.2021.710820

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Copyright © 2021 Ling, Wang, Ma, Xue, Gu, Cao and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Panx 1 and inflammatory cytokine expression in LPS-treated astrocytes. mRNA expression of Panx 1 (A) and the selected inflammatory cytokine IL-1β (D), IL-6 (E), IL-10 (F), TNF-α (G) and caspase 3 (H) were measured by qPCR assay. Primary cultured astrocytes were treated with LPS at different doses (100, 250, and 500 ng/ml) for 24 h. The levels were displayed as percentage of the control (the same volume of DMSO). The data were normalized to 18S mRNA levels. Western blot analysis of Panx 1 expression (B,C) after treatment with 100 ng/ml LPS. Protein levels were quantified and normalized to GAPDH levels, n = 3. All data are mean ± SEM. Statistical comparison for (A,D–H) were performed using one-way ANOVA, following by Newman–Keuls Multiple Comparison Test, and for (C) using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group. Student’s t-test.