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. 2021 Aug 12;15:710820. doi: 10.3389/fncel.2021.710820

FIGURE 5.

FIGURE 5

CBX inhibits LPS-induced inflammatory cytokine expression in primary cultured astrocytes. Analysis of the selected inflammatory cytokine mRNA expression by qPCR assay, including IL-1β (A), IL-6 (B), TNF-α (C), TGF-β (D), and IL-10 (E) in primary cultured astrocytes. Cells were pretreated with Panx 1 inhibitor CBX (100 mM, 60 min) before LPS treatment (100 ng/ml, 24 h) or be treated with CBX alone. The levels were displayed as percentage of the control (the same volume of DMSO). The data were normalized to 18S mRNA levels. (F–H) Evoked expression (content) of IL-1β, IL-6, and IL-10 in astrocytes following with different stimulation, n = 3. All data are mean ± SEM. Statistical comparison for were performed using one-way ANOVA, following by Newman–Keuls Multiple Comparison Test. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group.