FIGURE 7.

Effect of different conditioned medium on the viability of SY-SY5Y cells. (A) Illustration of the experimental design. After treatment with CBX or siRNA, astrocytes were incubated with 100 ng/ml LPS for 24 h. The medium we collected as ACM/LPS were added into SH-SY5Y cells for 24 h and CCK 8 assay was performed to estimate cell viability. (B) Impact of ACM/LPS on the viability of SH-SY5Y cells compared with the group of astrocyte conditioned medium + LPS per se (without treating astrocytes). (C) SH-SY5Y cell viability was assayed by CCK8 assay under different conditioned medium, which from cells pretreated with CBX at different doses before incubation with LPS in astrocytes. (D) SH-SY5Y cells were treated with CBX alone at different doses and the viability was measured by CCK8 assay. (E) The medium from astrocytes treated with Panx 1 siRNA + LPS were added into SH-SY5Y cells and cell viability was assayed by CCK8 assay. The data were normalized to ACM group levels (ACM + LPS per se), n = 3. All data are mean ± SEM. Statistical comparison for (C,D) were performed using one-way ANOVA, following by Newman–Keuls Multiple Comparison Test, and for (B,E) using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001 compared with ACM group.