FIGURE 1.

Changes in the key cholinergic enzymes in young and old Gdnf^wt/wt and Gdnf^wt/hyper mice. The activity of the acetylcholine-biosynthesizing enzyme, ChAT (A) and acetylcholine-degrading enzymes, AChE (B) and BuChE (C) measured in different brain regions of young (Y) and old (O) Gdnf^wt/wt and Gdnf^wt/hyper mice. The data are given as percent of mean values in young animals. The raw data is provided in the Supplementary File. (D) The corresponding changes in Cholinergic index in the Gdnf^wt/hyper mice and wild-type littermates. The Cholinergic index provides a measure between ACh biosynthesis by ChAT relative ACh-biodegradation by cholinesterases (AChE and BuChE). This index is calculated as the ratio between ChAT activity to the ChE activity. n = 10 per group (young) and n = 8 per group (old). Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. AChE, acetylcholinesterase; BuChE, butyrylcholinesterase; ChAT, choline acetyltransferase.