FIG. 2.

Baf-3 cells expressing dominant-negative NFIL3 require higher concentrations of IL-3 to survive. (A) Antibody-perturbed EMSA with lysates extracted from parental Baf-3 cells grown in 25 ng of IL-3 per ml (lane 1) and Baf-3 cells grown under the same conditions that express the dominant-negative NFIL3 protein (lane 2). The reaction mixture includes a ³²P-labeled oligonucleotide probe containing the NFIL3 binding sequence and anti-NFIL3 antiserum. The arrowhead indicates a supershifted complex containing NFIL3. (B) Parental Baf-3 cells and cells expressing the dominant-negative NFIL3 protein were cultured in serum-free AIM-V medium with recombinant murine IL-3 at the indicated concentrations for 36 h, after which the percentage of surviving cells was determined. Each value is the mean (± standard deviation) of three independent experiments.