FIG. 8.
Expression and phosphorylation of Akt in Baf-3 cells expressing Ras(G12V/V45E). Baf-3 cells containing Dex-inducible Ras(G12V/V45E) were cultured in IL-3-containing medium without Dex (lane 1) or with 10−7 M Dex for 16 h (lane 2). The Dex-treated cells were then cultured in IL-3-free, Dex-containing medium for 12 h without wortmannin (lane 3) or with 100 nM wortmannin (lane 4). Phosphorylated Akt at Ser-473 (upper panel) or total Akt (lower panel) protein was detected with antibodies specific for each protein.